The Axima CFR-plus is a matrix assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometer that provides both the high mass accuracy and the high resolution required for effective analysis of biomolecules in the field of proteomics.

Some of the applications include:

• Protein and peptide identification
• Nucleic acid analysis
• Rapid small molecule characterization
• Polymer analysis

Instrument Specs (Shimadzu)

Features and benefits of the Axima CFR-plus include:

• Mass range 1-500kDa in linear mode; 1-80kDa in reflectron mode
• Mass resolution 4000 Fwhm (insulin B chain) in linear mode; 20000 Fwhm (insulin) in reflectron mode
• Mass accuracy <5ppm in reflectron mode with internal calibration; 0.02% of the parent ion with PSD
• Precursor ion resolution 1 in 70
• Sensitivity 10fmol in reflectron mode (insulin B chain)
• New flexibility for CID fragmentation
• Automated sample target handling and analysis of various biochip formats
• Specialized automated software tools to accelerate and intelligently manage sample analysis

More information

See vendor's site: Shimadzu and the Axima CRF-plus brochure.

Protocols and procedures

Any manuals posted here are EXCLUSIVELY for the use related to the instruments housed in the BPL. All copyrights apply.

Suggested reading

• Database Searching for Protein Identification and Characterization
  http://www.matrixscience.com/pdf/asms_tutorial_2005.pdf
• Lennon, John J.
  Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry
  http://www.abrf.org/ABRFNews/1997/June1997/jun97lennon.html
• Terry DE, Umstot E, Desiderio DM.
  Optimized sample-processing time and peptide recovery for the mass spectrometric analysis of protein digests.
  J Am Soc Mass Spectrom. 2004 Jun;15(6):784-94.