|
|
Background and applications
The DeCyder software is used for 2D Difference Gel
Electrophoresis (2D DIGE) analysis. It significantly increases
throughput by accurately addressing measurement of protein
differences with statistical confidence and reduces hands-on
time from days to minutes with minimal user-to-user variation.
The software automatically detects, matches, and analyzes
protein spots in multiplexed fluorescent images, and is able to
detect small differences between protein spots with high
statistical confidence.
Applications include:
More information
See vendor's site:
GE-Healthcare
Suggested reading
- Lilley KS, Friedman DB. All about DIGE: quantification
technology for differential-display 2D-gel proteomics.
Expert Rev Proteomics. 2004 Dec;1(4):401-9.
- Friedman DB, Hill S, Keller JW, Merchant NB, Levy SE,
Coffey RJ, Caprioli RM. Proteome analysis of human
colon cancer by two-dimensional difference gel
electrophoresis and mass spectrometry.
Proteomics. 2004 Mar;4(3):793-811.
- Alban A, David SO, Bjorkesten L, Andersson C, Sloge E,
Lewis S, Currie I. 2003. A novel experimental design for
comparative two-dimensional gel analysis: two-dimensional
difference gel electrophoresis incorporating a pooled
internal standard. Proteomics. 3:36-44.
- Guest PC, Skynner HA, Salim K, Tattersall FD, Knowles MR,
Atack JR. 2006. Detection of gender differences in rat lens
proteins using 2-D-DIGE. Proteomics. 6:667-676.
- Tonge R, Shaw J, Middleton B, Rowlinson R, Rayner S,
Young J, Pognan F, Hawkins E, Currie I, Davison M. 2001.
Validation and development of fluorescence two-dimensional
differential gel electrophoresis proteomics technology.
Proteomics. 1:377-396.
- Unlu M, Morgan ME, Minden JS. 1997. Difference gel
electrophoresis: a single gel method for detecting changes
in protein extracts. Electrophoresis. 18:2071-2077.
- Yan JX, Devenish AT, Wait R, Stone T, Lewis S, Fowler S.
2002. Fluorescence two-dimensional difference gel
electrophoresis and mass spectrometry based proteomic
analysis of Escherichia coli. Proteomics. 2:1682-1698.
- Fujii K, Kondo T, Yokoo H, Okano T, Yamada M, Yamada T,
Iwatsuki K, Hirohashi S. 2006. Database of two-dimensional
polyacrylamide gel electrophoresis of proteins labeled with
CyDye DIGE Fluor saturation dye. Proteomics. Jan 23; [Epub
ahead of print].
- Swatton JE, Prabakaran S, Karp NA, Lilley KS, Bahn S.
2004.
- Protein profiling of human postmortem brain using
2-dimensional fluorescence difference gel electrophoresis
(2-D DIGE). Mol Psychiatry. 9:128-143. Review.
Protocols and procedures
Any manuals posted here are EXCLUSIVELY for the use related
to the instruments housed in the BPL. All copyrights apply.
|
