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2D gel electrophoresis resources include:
Ettan™ IPGphor™ II (GE-Healthcare)
 Ettan™ IPGphor™ isoelectric focusing (IEF) System performs first
dimension isoelectric focusing in the first dimension of 2D gel
electrophoresis. It runs up to 12 IPG strips (7, 11, 13, 18, or
24 cm) simultaneously using ceramic Ettan IPGphor manifold.
Sample cups can be used with Ettan IPGphor ceramic manifold to
significantly increase protein loads. The manifold is made of
aluminum oxide ceramic that exhibits exceptionally uniform
thermal characteristics. The high heat conductivity of the
ceramic material prevents any "hot spots" that can distort 2D
gel electrophoresis maps.
Ettan DALTsix Large Vertical System (GE-Healthcare)
This is a modular system for large format and medium throughput
2-D electrophoresis. The components include Ettan
DALTsix Electrophoresis Unit, Gel Caster and Gradient Maker, an
EPS 601 Power Supply, and MultiTemp™ III Thermostatic
Circulator. A built-in ceramic heat exchanger and buffer
circulation pump ensure efficient cooling. It runs up to six
large format (26 × 20 cm) second-dimension polyacrylamide gels
in 7 - 8 h. It runs faster at higher temperature (>15oC).
The system gels accommodate first dimension 17, 18- or 24-cm
Immobiline DryStrip gels (IPG strips) from both Ettan IPGphor™
and Multiphor™ II isoelectric focusing systems.
Rotofor (Bio-Rad)
 The
Rotofor cell is a unique preparative protein purification device
that separates/fractionates complex proteins samples in free
solution by liquid-phase isoelectric focusing (IEF).
Fractionation by liquid-phase IEF is particularly beneficial for
those proteins that are insoluble or otherwise do not separate
well in other, gel-based IEF media. Liquid-phase IEF enables
fractionation of proteins in their native state by isoelectric
point (pI). It offers rapid, simple, and effective fractionation
and purification of low-abundance proteins from complex protein
mixtures. The fractions from a run
can be easily harvested within specific pH intervals. Fractions
obtained with the Rotofor may be subjected to further
purification by gel electrophoresis, chromatography,
precipitation, etc.
The Rotofor system has a cylindrical focusing chamber, which is
available in different sizes – standard, mini, and micro. The
standard Rotofor chamber holds ca. 60 mL of sample, and is
commonly used to fractionate samples (a few mg to 4 g of total
protein) into 20 distinct fractions. The mini Rotofor chamber
holds 18 ml of sample and is ideal for small samples (µg to mg
protein) fractionation into 20 distinct fractions. The mini
Rotofor chamber is also ideal for refractionation of samples
harvested from a standard Rotofor run. The micro Rotofor
accommodates/processes 2.5 mL sample (µg to mg protein). It
separates samples into ten 200 – 500 µL fractions. It enables
500-fold purification of complex mixture of sample within 3 h; a
vacuum fraction collector instantly collects the resulting
fractions. Selected fractions from an initial run may be
collected, pooled, and refractionated, resulting in up to
1,000-fold purification.Experion (Bio-Rad)
The Experion is an automated 1D electrophoresis system. It
performs the multiple steps of gel-based electrophoresis
including separation, staining, destaining, band detection,
imaging, and data analysis. The Experion system produces rapid
highly reproducible separation and quantitation of protein or
RNA (either mRNA or total RNA) samples. The system requires very
little sample (ng - µg), with reproducible separation of protein
and RNA samples (10–12 samples simultaneously) in 30 minutes.
The Experion system components include Experion automated
electrophoresis station, Experion
priming station, Experion vortex station (for RNA applications)
and Experion software. The Experion system contains three
analysis kits: The Pro260 kit (separates and detects 2.5–2,000
ng/µl protein) for protein separation and analysis, the RNA
StdSens kit (detects 25–500 ng/µl RNA) for standard-sensitivity
RNA detection, and the RNA HighSens kit (200–5,000 pg/µl RNA)
for high-sensitivity RNA detection. The Experion chips require 4
µl of sample per well for protein analysis and 1 µl of sample
per well for RNA analysis. The sample wells can hold 4–10 µl
(sample, ladder, and destain), and the gel well holds 7–18 µl
gel matrix.
More information
See vendors' site:
GE_Healthcare
Bio-Rad
Protocols and procedures
Any manuals posted here are EXCLUSIVELY for the use related
to the instruments housed in the BPL. All copyrights apply.
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